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Meng-na-mei authored Oct 6, 2023
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Expand Up @@ -255,8 +255,8 @@ <h2>Construction of <i>hmaS</i> Mutant Library</h2>
positive clones
was 100%.</p>
<div class="figure-container" style="width: 80%;margin: 0 auto;">
<img src="../../images/图片1.png" alt="">
<p>gY9s-HmaS<sup>mut</sup>-Bio177 mutant library plates</p>
<img src="../../images/a4.jpg" alt="">

</div>

<p><b>Figure 1.</b> A mutant library with 10⁵ clones was constructed by using a
Expand Down Expand Up @@ -302,7 +302,7 @@ <h2>Construction and Test of a Dual-Module Screening Platform</h2>
concentrations, the selection pressure
accelerated, which enhanced the performance of viable individuals, improved HMA
production, and reduced fluorescence
strength. Based on colony fluorescence and HMA production, we screened clones under
intensity. Based on colony fluorescence and HMA production, we screened clones under
the conditions of 0.6 g/L HPP and
0.3 mg/L 5-FC (Fig. 3). And we found a mutant clone HmaS<sup>V152G</sup> that HMA
production of
Expand All @@ -321,8 +321,8 @@ <h2>Construction and Test of a Dual-Module Screening Platform</h2>
<img src="../../images/t8.jpg" alt="">
</div>
<p><b>Figure 4.</b> Graph of HMA production by the bacteria harboring
HmaS<sup>WT</sup>and
HmaS<sup>V152G</sup>clones. With 9 mM HPP as the substrate for
HmaS<sup>WT</sup> and
HmaS<sup>V152G</sup> clones. With 9 mM HPP as the substrate for
whole-cell catalysis, HMA accumulation by the gY9s-HmaS<sup>V152G</sup>-Bio177 and
gY9s-HmaS<sup>WT</sup>-Bio177 strains was monitored for 3
h.</p>
Expand Down Expand Up @@ -374,7 +374,7 @@ <h2>Engineering an <i>E. coli</i> Chassis with High-HPP Production</h2>
<p>we constructed the pSB1c-aroG<sup>fbr</sup>-tktA-ppsA (pATP) plasmid to
overexpress the
phosphoenol-pyruvate synthase (<i>ppsA</i>) gene,
the transketolase (<i>tktA</i>) gene and aro<i>G<sup>fbr</sup></i>gene.
the transketolase (<i>tktA</i>) gene and <i>aroG<sup>fbr</sup></i> gene.
Through the modifications of
genetic
overexpression, we obtained a host
Expand All @@ -400,7 +400,7 @@ <h2>Engineering an <i>E. coli</i> Chassis with High-HPP Production</h2>
<div class="figure-container" style="width: 60%;margin: 0 auto;">
<img src="../../images/26.jpg" alt="">
</div>
<p><b>Figure 7.</b> Bar graph of the Inhibitory efficiency by the random
<p><b>Figure 7.</b> Bar graph of the inhibitory efficiency by the random
double-mismatched eGFP-sgRNAs. Fi means fully interference,
transformed into the strain that containing the non-mutated R6K-dCas9-eGFP
plasmid, which had CA in the 7-8bp spacer of
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