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Saionjiko authored Oct 8, 2024
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76 changes: 76 additions & 0 deletions Article.html
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<!DOCTYPE html>
<html lang="en">
<head>
<meta charset="UTF-8">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
<title>Article</title>

<link rel="icon" type="image/png" href="./images/team logo.png" sizes="16x16">

<link rel="stylesheet" href="css/reset.css">
<link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.5/css/bootstrap.min.css">
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<script src="https://ajax.googleapis.com/ajax/libs/jquery/3.5.1/jquery.min.js"></script>
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<script src="js/granim.js"></script>
<script src="js/wow.min.js"></script>
<script src="js/script.js"></script>


</head>
<body>
<!-- 导航栏 -->
<div class="container-fluid">
<nav class="navbar navbar-inverse">
<div class="container-fluid">
<ul class="nav navbar-nav">
<li><a id="len1" class="hoverable" href="index.html">HOME</a></li>
<li><a id="len2" class="hoverable" href="Project.html">Project</a></li>
<li><a id="len3" class="hoverable" href="Article.html" style="color:white">Article</a></li>
<li><a id="len5" class="hoverable" href="Team.html">Team</a></li>
</ul>
</div>
</nav>
</div>
<!-- END -->

<div id="logo">
<img src="./images/team logo.png" alt="TeamLogo">
</div>

<!-- 文章主体 -->
<section class="candy-wrapper">

<div class="tabs">
<input type="radio" id="tab1" name="tab-control" checked>
<input type="radio" id="tab2" name="tab-control">

<ul>
<li title="Features"><label for="tab1" role="button"><svg t="1728057861497" class="icon" viewBox="0 0 1024 1024" version="1.1" xmlns="http://www.w3.org/2000/svg" p-id="4283"><path d="M661.648 118H249.104c-39.088 0-70.304 31.752-70.304 70.304v647.392c0 39.096 31.752 70.304 70.304 70.304h525.8c38.552 0 70.296-31.752 70.296-70.304V301.56L661.648 118z m12.664 74.704l96.176 96.184H684.584c-5.408 0-10.272-4.864-10.272-10.272V192.704z m126.928 642.992a26.24 26.24 0 0 1-26.344 26.352H249.104a26.24 26.24 0 0 1-26.344-26.352V188.304a26.24 26.24 0 0 1 26.344-26.344v-0.464h381.328v117.192c0 29.824 24.416 54.16 54.16 54.16h116.656v502.848z" p-id="4284"></path><path d="M348.68 386.072h188.968a21.84 21.84 0 0 0 21.944-21.936 21.84 21.84 0 0 0-21.944-21.944H348.68a21.832 21.832 0 0 0-21.944 21.944 21.84 21.84 0 0 0 21.944 21.936zM674.848 644.8H348.68c-12.208 0-21.944 9.744-21.944 21.944s9.736 21.944 21.944 21.944h326.168c12.208 0 21.944-9.744 21.944-21.944S687.064 644.8 674.848 644.8zM348.68 495.856a21.824 21.824 0 0 0-21.944 21.944 21.832 21.832 0 0 0 21.944 21.936h326.168a21.832 21.832 0 0 0 21.944-21.936 21.832 21.832 0 0 0-21.944-21.944H348.68z" p-id="4285"></path></svg><br><span>Report</span></label></li>

<li title="Delivery Contents"><label for="tab2" role="button"><svg viewBox="0 0 24 24"><path d="M2,10.96C1.5,10.68 1.35,10.07 1.63,9.59L3.13,7C3.24,6.8 3.41,6.66 3.6,6.58L11.43,2.18C11.59,2.06 11.79,2 12,2C12.21,2 12.41,2.06 12.57,2.18L20.47,6.62C20.66,6.72 20.82,6.88 20.91,7.08L22.36,9.6C22.64,10.08 22.47,10.69 22,10.96L21,11.54V16.5C21,16.88 20.79,17.21 20.47,17.38L12.57,21.82C12.41,21.94 12.21,22 12,22C11.79,22 11.59,21.94 11.43,21.82L3.53,17.38C3.21,17.21 3,16.88 3,16.5V10.96C2.7,11.13 2.32,11.14 2,10.96M12,4.15V4.15L12,10.85V10.85L17.96,7.5L12,4.15M5,15.91L11,19.29V12.58L5,9.21V15.91M19,15.91V12.69L14,15.59C13.67,15.77 13.3,15.76 13,15.6V19.29L19,15.91M13.85,13.36L20.13,9.73L19.55,8.72L13.27,12.35L13.85,13.36Z" />
</svg><br><span>Supplementary</span></label></li>

</ul>

<div class="content">
<section>
<embed src="assets/正文.pdf" class="pdf-viewer"></embed>
</section>

<section>
<embed src="assets/支持信息.pdf"class="pdf-viewer"></embed>
</section>
</div>

</div>
</section>


<footer>
<p>sonsor</p>
</footer>

</body>
</html>
90 changes: 90 additions & 0 deletions Project.html
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<!DOCTYPE html>
<html lang="en">
<head>
<meta charset="UTF-8">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
<title>Project</title>

<link rel="icon" type="image/png" href="./images/team logo.png" sizes="16x16">

<link rel="stylesheet" href="css/reset.css">
<link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.5/css/bootstrap.min.css">
<link rel="stylesheet" href="css/Article.css">

<script src="https://ajax.googleapis.com/ajax/libs/jquery/3.5.1/jquery.min.js"></script>
<script src="js/skrollr.min.js"></script>
<script src="js/granim.js"></script>
<script src="js/wow.min.js"></script>
<script src="js/script.js"></script>


</head>
<body>
<!-- 导航栏 -->
<div class="container-fluid">
<nav class="navbar navbar-inverse">
<div class="container-fluid">
<ul class="nav navbar-nav">
<li><a id="len1" class="hoverable" href="index.html">HOME</a></li>
<li><a id="len2" class="hoverable" href="Project.html" style="color:white">Project</a></li>
<li><a id="len3" class="hoverable" href="Article.html">Article</a></li>
<li><a id="len5" class="hoverable" href="Team.html">Team</a></li>
</ul>
</div>
</nav>
</div>
<!-- END -->

<div id="logo">
<img src="./images/team logo.png" alt="TeamLogo">
</div>

<!-- 文章主体 -->
<section class="candy-wrapper">

<div class="tabs">
<input type="radio" id="tab1" name="tab-control" checked>
<input type="radio" id="tab2" name="tab-control">

<ul>
<li title="Features"><label for="tab1" role="button"><svg t="1727963802891" class="icon" viewBox="0 0 1261 1024" version="1.1" xmlns="http://www.w3.org/2000/svg" p-id="4325" width="1em"><path d="M1181.538462 1024H78.769231a78.769231 78.769231 0 0 1-78.769231-78.769231V78.769231a78.769231 78.769231 0 0 1 78.769231-78.769231h1102.769231a78.769231 78.769231 0 0 1 78.76923 78.769231v866.461538a78.769231 78.769231 0 0 1-78.76923 78.769231zM95.310769 929.476923h1071.261539V94.523077H95.310769z" p-id="4326"></path><path d="M512.787692 918.449231l-207.950769-283.569231-253.636923 214.252308-51.2-60.652308L318.227692 519.876923l200.073846 273.329231 391.483077-439.532308 344.221539 498.609231-64.590769 44.110769-287.507693-415.113846-389.12 437.169231z" p-id="4327"></path><path d="M355.249231 393.846154A118.153846 118.153846 0 1 1 472.615385 275.692308 118.153846 118.153846 0 0 1 355.249231 393.846154z m0-173.292308a55.138462 55.138462 0 1 0 55.138461 55.138462 55.138462 55.138462 0 0 0-55.138461-55.138462z" p-id="4328"></path></svg><br><span>Poster</span></label></li>

<li title="Delivery Contents"><label for="tab2" role="button"><svg viewBox="0 0 24 24"><path d="M2,10.96C1.5,10.68 1.35,10.07 1.63,9.59L3.13,7C3.24,6.8 3.41,6.66 3.6,6.58L11.43,2.18C11.59,2.06 11.79,2 12,2C12.21,2 12.41,2.06 12.57,2.18L20.47,6.62C20.66,6.72 20.82,6.88 20.91,7.08L22.36,9.6C22.64,10.08 22.47,10.69 22,10.96L21,11.54V16.5C21,16.88 20.79,17.21 20.47,17.38L12.57,21.82C12.41,21.94 12.21,22 12,22C11.79,22 11.59,21.94 11.43,21.82L3.53,17.38C3.21,17.21 3,16.88 3,16.5V10.96C2.7,11.13 2.32,11.14 2,10.96M12,4.15V4.15L12,10.85V10.85L17.96,7.5L12,4.15M5,15.91L11,19.29V12.58L5,9.21V15.91M19,15.91V12.69L14,15.59C13.67,15.77 13.3,15.76 13,15.6V19.29L19,15.91M13.85,13.36L20.13,9.73L19.55,8.72L13.27,12.35L13.85,13.36Z" />
</svg><br><span>introduction</span></label></li>

</ul>

<div class="content">
<section>
<img class="poster" src="images/poster.jpg" alt="poster">
</section>

<section>
<article class="main">
<h1>Dry lab</h1>
<p>The thermal stability of biomolecules obtained by ancestral sequence reconstruction is typically higher. In order to achieve this, we reconstructed the phylogenetic tree of TcCGT1. The 11 proteins obtained through homology modeling were subjected to screening based on the AVE_RMSF of a given region at varying temperatures, binding free energy, and 8-C glycosylation energy barriers . It is anticipated that the final screened progenitor protein, ASR1, will retain some thermal stability and catalytic activity.

<p>To investigate the catalytic ability of ASR1, the C/O-glycosylation barriers were calculated at the DFT level. Additionally, the results of QM/MM simulations suggest that ASR1 may exhibit similarities with TcCGT1.</p>

<h1>Wet lab</h1>

<p>
For the sequences obtained after iterative ASR, we constructed plasmids and screened the novel proteases through heterologous expression in E. coli vectors, and we put the purified proteins into in vitro reactions and measured their reactivity under different environments (pH, temperature, etc.) to evaluate the strengths and weaknesses of the proteins, and together with the predicted favourable mutation sites in the previous stage, we can modify the proteins twice through targeted mutagenesis to obtain the the desired protein
</p>


</article>

</section>
</div>

</div>

</section>

<footer>
<p>sonsor</p>
</footer>

</body>
</html>
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