Update results.md

docs/project/results.md

@@ -70,6 +70,7 @@ Both hypotheses lead to gIII expression independent of the E3 ligase activity. T
 <figcaption> Phage propagation in the presence and absence of split-RNAP components. This figure shows how much the phage population changed after overnight incubation with bacteria missing one of the two halves of the split-RNAP. The results are shown as a log2-fold change, meaning the numbers show how much the phage count increased or decreased, compared to the starting level. NC (negative control) is a bacterial strain lacking both parts of the system. PC (positive control) is a bacterial strain with the full system.</figcaption>
 </figure>
 
+### How can we reduce unwanted, E3-ligase independent page propagation? 
 We observed that the RNAP enzyme can assemble and become active before the phages even enter the bacterial cells. This early assembly can lead to unwanted phage propagation, which interferes with our goal to link phage propagation to E3 ligase activity. To overcome this problem, we changed the way we control the production of the N- and C-terminal halves of the RNAP. Instead of using a constitutive promoter, which is always active and produces the protein, we switched to a promoter that can be turned on when needed. For this, we chose two different inducible systems. One is switched on by adding vanillic acid (pVan promoter), the other by the stress response caused by phage infection (phage shock promoter). These new systems let us delay the production of the RNAP halves until just before or during phage infection. By regulating when these components are expressed, we can reduce the unintended assembly of the RNAP. This method can easily be added to the PACE system, allowing precise control of the timing of RNAP production.
 
 ### Enhancing SIAH1 genetic diversity using drift.