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Dorado demux getting error with bam files #1161

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wasifakabir opened this issue Dec 4, 2024 · 2 comments
Closed

Dorado demux getting error with bam files #1161

wasifakabir opened this issue Dec 4, 2024 · 2 comments

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@wasifakabir
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wasifakabir commented Dec 4, 2024

##Dorado demux getting error with bam files##

I ran dorado basecall with SUP with barcode classification. I am trying to demux them now with --no-classify option. But I kept getting an error saying the following
"Illegal instruction (core dumped)".
I only get this error when I am trying to use BAM files as input. I dont get the error if I use FASTQ file.
Following is the script-
#!/bin/bash
#SBATCH --account=XXXXX
#SBATCH --job-name=dorado_demux
#SBATCH --partition=a100 --gpus-per-node=2
#SBATCH --ntasks-per-node=2 --nodes=1
#SBATCH --mem-per-cpu=32G
#SBATCH --time=01:00:00

module load dorado/0.5.1-foss-2022a-CUDA-11.7.0

Do some work:
DATADIR="xxx/011024_sup_bam_classified"
OUTDIR="xxx/011024_sup_bam_classified/demux"
dorado demux --no-classify --emit-fastq --output-dir "${OUTDIR}" "${DATADIR}"/calls_01102024_class_61_72.bam
exit 0

@wasifakabir wasifakabir changed the title Hi, Dorado demux getting error with bam files Dec 4, 2024
@malton-ont
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Hi @wasifakabir,

It looks like you are using dorado-0.5.1 - can you try with the most recent version? If the issue still persists in a later version, are you able to provide us with an example BAM file? Can you also give us some information about the system you are running on?

@malton-ont
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Closing due to inactivity. If this issue persists, please re-open.

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