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Prettier linting
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DLBPointon committed Aug 8, 2024
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Expand Up @@ -49,7 +49,6 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d

YamlInput parses the input yaml into channels for later use in the pipeline.


### Validate TaxID

<details markdown="1">
Expand All @@ -61,7 +60,6 @@ YamlInput parses the input yaml into channels for later use in the pipeline.

Validate TaxID scans through the taxdump to ensure that the input taxid is present in the nxbi taxdump.


### Filter Fasta

<details markdown="1">
Expand All @@ -74,7 +72,6 @@ Validate TaxID scans through the taxdump to ensure that the input taxid is prese

By default scaffolds above 1.9Gb are removed from the assembly, as scaffolds of this size are unlikely to truely have contamination. There is also the issue that scaffolds larger than this use a significant amount of resources which hinders production environments.


### GC Content

<details markdown="1">
Expand All @@ -87,7 +84,6 @@ By default scaffolds above 1.9Gb are removed from the assembly, as scaffolds of

Calculating the GC content of the input genome.


### Generate Genome

<details markdown="1">
Expand All @@ -100,7 +96,6 @@ Calculating the GC content of the input genome.

An index-like file containing the scaffold and scaffold length of the input genome.


### Trailing Ns Check

<details markdown="1">
Expand All @@ -113,7 +108,6 @@ An index-like file containing the scaffold and scaffold length of the input geno

A text file containing a report of the Ns found in the genome.


### Get KMERS profile

<details markdown="1">
Expand All @@ -126,7 +120,6 @@ A text file containing a report of the Ns found in the genome.

A csv file containing kmers and their counts.


### Extract Tiara Hits

<details markdown="1">
Expand All @@ -141,7 +134,6 @@ A csv file containing kmers and their counts.

Tiara ...


### Mito Organellar Blast

<details markdown="1">
Expand All @@ -154,7 +146,6 @@ Tiara ...

A BlastN based subworkflow used on the input genome to filter potential contaminants from the genome.


### Chloro Organellar Blast

<details markdown="1">
Expand All @@ -167,7 +158,6 @@ A BlastN based subworkflow used on the input genome to filter potential contamin

A BlastN based subworkflow used on the input genome to filter potential contaminants from the genome.


### Run FCS Adaptor

<details markdown="1">
Expand All @@ -184,7 +174,6 @@ A BlastN based subworkflow used on the input genome to filter potential contamin

FCS Adaptor Identified potential locations of retained adaptor sequences from the sequencing run.


### Run FCS-GX

<details markdown="1">
Expand All @@ -198,7 +187,6 @@ FCS Adaptor Identified potential locations of retained adaptor sequences from th

FCS-GX Identified potential locations of contaminant sequences.


### Pacbio Barcode Check

<details markdown="1">
Expand All @@ -211,7 +199,6 @@ FCS-GX Identified potential locations of contaminant sequences.

Uses BlastN to identify where given barcode sequences may be in the genome.


### Run Read Coverage

<details markdown="1">
Expand All @@ -225,7 +212,6 @@ Uses BlastN to identify where given barcode sequences may be in the genome.

Mapping the read data to the input genome and calculating the average coverage across it.


### Run Vecscreen

<details markdown="1">
Expand All @@ -238,7 +224,6 @@ Mapping the read data to the input genome and calculating the average coverage a

Vecscreen identifies vector contamination in the input sequence.


### Run NT Kraken

<details markdown="1">
Expand All @@ -256,7 +241,6 @@ Vecscreen identifies vector contamination in the input sequence.

Kraken assigns taxonomic labels to metagenomic DNA sequences and optionally outputs the fastq of these data.


### Nucleotide Diamond Blast

<details markdown="1">
Expand All @@ -273,7 +257,6 @@ Kraken assigns taxonomic labels to metagenomic DNA sequences and optionally outp

Diamond Blast is a sequence aligner for translated and protein sequences, here it is used do identify contamination usin the NCBI db


### Uniprot Diamond Blast

<details markdown="1">
Expand All @@ -290,7 +273,6 @@ Diamond Blast is a sequence aligner for translated and protein sequences, here i

Diamond Blast is a sequence aligner for translated and protein sequences, here it is used do identify contamination usin the Uniprot db


### Create BTK dataset

<details markdown="1">
Expand All @@ -304,7 +286,6 @@ Diamond Blast is a sequence aligner for translated and protein sequences, here i

Create BTK, creates a BTK_dataset folder compatible with BTK viewer.


### Autofilter and check assembly

<details markdown="1">
Expand All @@ -320,7 +301,6 @@ Create BTK, creates a BTK_dataset folder compatible with BTK viewer.

Autofilter and check assembly returns a decontaminated genome file as well as summaries of the contamination found.


### Generate samplesheet

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Expand All @@ -333,7 +313,6 @@ Autofilter and check assembly returns a decontaminated genome file as well as su

This produces a CSV containing information on the read data for use in BlobToolKit.


### Sanger-TOL BTK

<details markdown="1">
Expand All @@ -351,7 +330,6 @@ This produces a CSV containing information on the read data for use in BlobToolK

Sanger-Tol/BlobToolKit is a Nextflow re-implementation of the snakemake based BlobToolKit pipeline and produces interactive plots used to identify true contamination and seperate sequence from the main assembly.


### Merge BTK datasets

<details markdown="1">
Expand All @@ -365,7 +343,6 @@ Sanger-Tol/BlobToolKit is a Nextflow re-implementation of the snakemake based Bl

This module merged the Create_btk_dataset folder with the Sanger-tol BTK dataset to create one unified dataset for use with btk viewer.


### ASCC Merge Tables

<details markdown="1">
Expand All @@ -380,7 +357,6 @@ This module merged the Create_btk_dataset folder with the Sanger-tol BTK dataset

Merge Tables merged the summary reports from a number of modules inorder to create a single set of reports.


### Pipeline information

<details markdown="1">
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