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## Frequently Asked Questions ## | ||
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1. When does WALT get much lower mapping rate (mapping effiency) compared to other bisulfite mappers? | ||
By default, WALT ignores reads shorter than 39. If you get lower mapping rate, please go to mapstats file and check the percentage of reads that are shorter than 39. If a large portion of reads are shorter than 39, I suggest you use WALT seed pattern 7. | ||
(1) Open walt/src/walt/Makefile, modify “-D SEEDPATTERN3" to " -D SEEDPATTERN7" | ||
(2) rebuild the program using make all, make install | ||
(3) rebuild your index using the new makedb command | ||
(4) mapping reads using the new walt command | ||
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By default, WALT ignores reads shorter than 39. If you get lower mapping rate, please go to mapstats file and check the percentage of reads that are shorter than 39. If a large portion of reads are shorter than 39, I suggest you use WALT seed pattern 7. | ||
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(1) Open walt/src/walt/Makefile, modify “-D SEEDPATTERN3" to " -D SEEDPATTERN7" | ||
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(2) rebuild the program using make all, make install | ||
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(3) rebuild your index using the new makedb command | ||
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(4) mapping reads using the new walt command | ||
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