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README file for ddPCR

201607, HFvanEssen

Use ddPCR QuantaSoft software to export raw Amplitude data

  • 'Export Amplitude and Cluster Data' in options

Pipeline example:

  • createDesign
  • loop through individual targets
  • combine all samples in each used probe
  • find outliers
  • find global thresholds combined data
  • plot combined data with basic thresholds
  • refine global thresholds for combined data
  • plot combined data with refined thresholds
  • loop through each sample for each target with refined threshold
  • plot data
  • use calculateStats... functions to get sample statistics

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