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tobiasrausch committed Jan 26, 2024
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# Wally

Plotting of aligned sequencing reads, assembled contigs or pan-genome graphs in BAM/CRAM/GFA format and visualization of genomic variants.
Plotting of aligned sequencing reads, assembled contigs or pan-genome graphs in BAM/CRAM format and visualization of genomic variants.

## Installing Wally

Expand Down Expand Up @@ -171,19 +171,6 @@ To visualize a self-alignment of a read with reference mappings use `-a`

`wally dotplot -a -r read_name -g hg38.fa input.bam`

## Subcommand `gfa`: Visualization of pan-genome graphs (work-in-progress)

Pan-genome graphs in [rGFA](https://github.com/lh3/gfatools/blob/master/doc/rGFA.md#the-reference-gfa-rgfa-format) format can be visualized using

`wally gfa -g GRCh38.fa example/example.gfa.gz`

With [gfatools](https://github.com/lh3/gfatools), you can extract subgraphs out of larger [prebuilt human graphs](https://github.com/lh3/minigraph#prebuilt-graphs).

`gfatools view -R chr6:31354483-31357158 -r 2 GRCh38-90c.r518.gfa | bgzip > subgraph.gfa.gz`

`wally gfa -g GRCh38.fa subgraph.gfa.gz`


## Citation

Tobias Rausch, Rene Snajder, Adrien Leger, Milena Simovic, Mădălina Giurgiu, Laura Villacorta, Anton G. Henssen, Stefan Fröhling, Oliver Stegle, Ewan Birney, Marc Jan Bonder, Aurelie Ernst, Jan O. Korbel
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