Dr. Denis Tsygankov (2021)
Integrative Systems Biology Lab
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University SOM
If you use any part of the scripts in this package, please cite:
DJ Marston et al. "Correcting artifacts in ratiometric biosensor imaging; an improved approach for dividing noisy signals" Frontiers in Cell and Developmental Biology (2021), doi: 10.3389/fcell.2021.685825
The package includes two examples of data sets (from GEF Asef and CDC42 biosensors):
Numerator1.tif (a multi frame signal for the numerator of the ratio)
Denominator1.tif (a multi frame signal for the denominator of the ratio)
Mask1.tif (a single frame image of the cell mask)
and similarly:
Numerator2.tif
Denominator2.tif
Mask2.tif
The scripts NCF_processing1.m and NCF_processing2.m illustrate the NCF method using the optimizations defined by Equations 5 and 6 in the paper, respectively.
If you use NCF_processing1.m for the first data set and NCF_processing2.m for the second data set, you will see that results that correspond to Figures 8 and 9, respectively.
The package also includes profile.m, which was used to generate the profiles of the mean intensity along the cell perimeter as a function of the distance from the cell edge for Figures 1, 3, 4 and 6 in the paper.