SPACED (Spatial Classification of mRNA Expression Data) analysis is a computational method to infer spatial organization of celltypes identified in scRNA-seq data. This method requires the following parameters as input:
1.obj -> seurat object
2.degs -> differential expressed genes
3.ident_use -> cell identity class
4.celltype -> cell identity
SPACED exhibited greater performance with the top10 most cluster specific genes
SPACED is restricted to the brain regions which have clear anatomic structures and distinguishable borders