Skip to content

Commit

Permalink
Sarah's comments #44
Browse files Browse the repository at this point in the history
  • Loading branch information
strambc committed Sep 10, 2024
1 parent 24b4b9c commit 6d9127c
Show file tree
Hide file tree
Showing 2 changed files with 3 additions and 3 deletions.
4 changes: 2 additions & 2 deletions docs/source/intro.rst
Original file line number Diff line number Diff line change
Expand Up @@ -17,9 +17,9 @@ FISH Omics techniques description
Figure 1: FISH omics methods can be utilized to map chromatin structures across multiple genomic scales (Figure credit: Sarah Aufmkolk; `Dekker et al., 2023, Mol.Cell <https://doi.org/10.1016/j.molcel.2023.06.018>`_.


Specific genomic sequences can be visualized by fluorescence in situ hybridization (FISH) using fluorescently labeled DNA probes, and their location in individual cells can be imaged using either traditional microscopy techniques (e.g., widefield or confocal)or super-resolution microscopy as reviewed by (`Fraser et al. <https://doi.org/10.1128/MMBR.00006-15>`_) and (`Jerkovic and Cavilli <https://doi.org/10.1038/s41580-021-00362-w>`_)
Specific genomic sequences can be visualized by fluorescence in situ hybridization (FISH) using fluorescently labeled DNA probes, and their location in individual cells can be imaged using either traditional microscopy techniques (e.g., widefield or confocal) or super-resolution microscopy as reviewed by (`Fraser et al. <https://doi.org/10.1128/MMBR.00006-15>`_) and (`Jerkovic and Cavilli <https://doi.org/10.1038/s41580-021-00362-w>`_).

As a logical evolution of more traditional techniques allow the detection of multiple and ideally all DNA locations across the genome (see recent reviews: `Jercovic and Cavalli <https://doi.org/10.1038/s41580-021-00362-w>`_, `Boettiger and Murphy <https://doi.org/10.1016/j.tig.2019.12.010>`_, `Hu and Wang <https://doi.org/10.1016/j.tcb.2020.10.006>`_, `Maslova and Krasikova <https://doi.org/10.3389/fcell.2021.753097>`_,
Evolutions of these techniques expanded the number of imaged targets to multiple and potentially all DNA locations across the genome. (see recent reviews: `Jercovic and Cavalli <https://doi.org/10.1038/s41580-021-00362-w>`_, `Boettiger and Murphy <https://doi.org/10.1016/j.tig.2019.12.010>`_, `Hu and Wang <https://doi.org/10.1016/j.tcb.2020.10.006>`_, `Maslova and Krasikova <https://doi.org/10.3389/fcell.2021.753097>`_,
`Zhuang <https://doi.org/10.1038/s41592-020-01037-8>`_, and `Bouwman et al. <https://doi.org/10.1016/j.molcel.2023.06.018>`_).

Collectively these technologies can be called interchangeably **multiplexed FISH** or **FISH omics**, which emphasize the visualization of multiple or ideally all genomic targets, respectively. These methods provide an expanded understanding of how higher-order chromosome structure relates to transcriptional activity and cell development.
Expand Down
2 changes: 1 addition & 1 deletion docs/source/trace.rst
Original file line number Diff line number Diff line change
Expand Up @@ -50,7 +50,7 @@ Example
-------
The only mandatory column in this table is ``Trace_ID``. All other columns are optional and must be defined by the user using a Header line starting with ``#^``.

.. tip:: the Optional columns in this example table are included for illustrative purposes only and describe a case in which the user is reporting the **allele** to which each trace was mapped, the **intensity** of the nascent RNA expression signal associated with each trace and the **distance** of each trace from the nuclear lamina.
.. tip:: the optional columns in this example table are included for illustrative purposes only and describe a case in which the user is reporting the **allele** to which each trace was mapped, the **intensity** of the nascent RNA expression signal associated with each trace and the **distance** of each trace from the nuclear lamina.

.. include:: examples/trace
:code:

0 comments on commit 6d9127c

Please sign in to comment.