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Merge pull request #2 from claraliu97/main
Adding sn processing scripts
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#Basic commands to run cellranger for single-nuclei Multiome-sequencing Data | ||
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#References: https://support.10xgenomics.com/single-cell-multiome-atac-gex/software/pipelines/latest/what-is-cell-ranger-arc | ||
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#Reference Genome:https://support.10xgenomics.com/single-cell-multiome-atac-gex/software/downloads/latest? | ||
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#Sample_ID | ||
sample='' | ||
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#Path to reference | ||
reference='refdata-cellranger-arc-GRCh38-2020-A-2.0.0' | ||
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#Path to libraries | ||
libraries='library.csv' | ||
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cellranger-arc-2.0.0/cellranger-arc count --id $sample --reference $reference --libraries $libraries --disable-ui --localcores 12 --localmem 100 |
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#Basic commands to run cellranger for single-cell or single-nuclei RNA-sequencing Data | ||
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#References: https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger | ||
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#Reference Genome: https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest | ||
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#Sample_ID | ||
sample='' | ||
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#Path to reference | ||
reference='refdata-gex-GRCh38-2020-A' | ||
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#Path to FASTQ files | ||
fastq_dir='FASTQ/' | ||
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cellranger-6.0.2/cellranger count --id $sample --fastqs $fastq_dir --localmem=300 --localcores=40 --transcriptome $reference |