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Merge pull request #2 from claraliu97/main
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Adding sn processing scripts
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simoncmo authored Jun 27, 2024
2 parents 8a7cbbd + fa7b747 commit 97a835b
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16 changes: 16 additions & 0 deletions Processing_script/cellranger-arc.v.2.0.0.sh
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#Basic commands to run cellranger for single-nuclei Multiome-sequencing Data

#References: https://support.10xgenomics.com/single-cell-multiome-atac-gex/software/pipelines/latest/what-is-cell-ranger-arc

#Reference Genome:https://support.10xgenomics.com/single-cell-multiome-atac-gex/software/downloads/latest?

#Sample_ID
sample=''

#Path to reference
reference='refdata-cellranger-arc-GRCh38-2020-A-2.0.0'

#Path to libraries
libraries='library.csv'

cellranger-arc-2.0.0/cellranger-arc count --id $sample --reference $reference --libraries $libraries --disable-ui --localcores 12 --localmem 100
17 changes: 17 additions & 0 deletions Processing_script/cellranger.v5.0.1.sh
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#Basic commands to run cellranger for single-cell or single-nuclei RNA-sequencing Data

#References: https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger

#Reference Genome: https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest

#Sample_ID
sample=''

#Path to reference
reference='refdata-gex-GRCh38-2020-A'

#Path to FASTQ files
fastq_dir='FASTQ/'


cellranger-6.0.2/cellranger count --id $sample --fastqs $fastq_dir --localmem=300 --localcores=40 --transcriptome $reference

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