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Merge pull request #76 from griffithlab/myhoang/Installation_page
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update Installation doc so the packages ver are same as aws set up pa…
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mhoang22 authored Nov 14, 2023
2 parents 47d59bb + 40d46fd commit 343f652
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33 changes: 15 additions & 18 deletions _posts/0000-10-01-Installation.md
Original file line number Diff line number Diff line change
Expand Up @@ -48,7 +48,7 @@ Installation of the bam-readcount tool involves "cloning" the source code with a

```bash
cd $RNA_HOME/student_tools/
export SAMTOOLS_ROOT=$RNA_HOME/student_tools/samtools-1.16.1
export SAMTOOLS_ROOT=$RNA_HOME/student_tools/samtools-1.18
git clone https://github.com/genome/bam-readcount
cd bam-readcount
mkdir build
Expand Down Expand Up @@ -81,9 +81,9 @@ The `stringtie` reference guided transcript assembly and abundance estimation to

```bash
cd $RNA_HOME/student_tools/
wget http://ccb.jhu.edu/software/stringtie/dl/stringtie-2.1.6.Linux_x86_64.tar.gz
tar -xzvf stringtie-2.1.6.Linux_x86_64.tar.gz
cd stringtie-2.1.6.Linux_x86_64
wget http://ccb.jhu.edu/software/stringtie/dl/stringtie-2.2.1.tar.gz
tar -xzvf stringtie-2.2.1.tar.gz
cd stringtie-2.2.1
make release
./stringtie -h
```
Expand Down Expand Up @@ -234,12 +234,11 @@ Installation type: download precompiled binary. Citation: [PMID: 22576172](https

```bash
cd $RNA_HOME/student_tools/
mkdir bedops_linux_x86_64-v2.4.40
cd bedops_linux_x86_64-v2.4.40
wget -c https://github.com/bedops/bedops/releases/download/v2.4.40/bedops_linux_x86_64-v2.4.40.tar.bz2
tar -jxvf bedops_linux_x86_64-v2.4.40.tar.bz2
mkdir bedops_linux_x86_64-v2.4.41
cd bedops_linux_x86_64-v2.4.41
wget -c https://github.com/bedops/bedops/releases/download/v2.4.41/bedops_linux_x86_64-v2.4.41.tar.bz2
tar -jxvf bedops_linux_x86_64-v2.4.41.tar.bz2
./bin/bedops
./bin/gff2bed
```

## [gtfToGenePred](https://bioconda.github.io/recipes/ucsc-gtftogenepred/README.html)
Expand Down Expand Up @@ -271,7 +270,7 @@ chmod a+x genePredToBed
## [how_are_we_stranded_here](https://github.com/betsig/how_are_we_stranded_here)

```bash
pip3 install git+https://github.com/betsig/how_are_we_stranded_here.git
pip3 install git+https://github.com/kcotto/how_are_we_stranded_here.git
check_strandedness
```

Expand All @@ -282,20 +281,18 @@ check_strandedness
```bash
cd $RNA_HOME/student_tools/
wget `download_link`
tar -xzvf cellranger-6.1.2.tar.gz
tar -xzvf cellranger-7.2.0.tar.gz
```

## Install [R](http://www.r-project.org/)

```bash
#sudo apt-get remove r-base-core
#sudo apt-get remove r-base
#wget -c https://cran.r-project.org/src/base/R-4/R-4.0.0.tar.gz
#tar -xf R-4.0.0.tar.gz
#cd R-4.0.0
#./configure
#make -j9
#sudo make install

#wget -qO- https://cloud.r-project.org/bin/linux/ubuntu/marutter_pubkey.asc | sudo gpg --dearmor -o /usr/share/keyrings/r-project.gpg
#echo "deb [signed-by=/usr/share/keyrings/r-project.gpg] https://cloud.r-project.org/bin/linux/ubuntu jammy-cran40/" | sudo tee -a /etc/apt/sources.list.d/r-project.list
#sudo apt update
#sudo apt install --no-install-recommends r-base
```

Note, if X11 libraries are not available you may need to use `--with-x=no` during config, on a regular linux system you would not use this option.
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