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UFITS v0.3.2
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@nextgenusfs nextgenusfs released this 23 Mar 20:55
· 461 commits to master since this release
0.3.2
c83112e

A somewhat major bug fix: the de-multiplexing script for Ion Torrent data was not removing reverse primer correctly. This has been fixed.

  • complete re-write of the ufits filter script to now normalize counts per number of reads in each sample prior to running the --index_bleed filter to remove noise from the dataset.
  • ufits filter script now can deal with synthetic mock spike-in control (set as default). Calculates index-bleed in both directions and smartly filters OTU table. (email me for details on synthetic mock if you are interested).
  • Taxonomy database was updated to most recent UNITE release
  • Taxonomy database installation method is changed, now downloads pre-formatted databases for ITS1, ITS2, and FULL length ITS sets.
  • Updated UCHIME reference sequences
  • New functionality for ufits taxonomy that allows for removal of non-fungal OTUs with the --only_fungi argument
  • Added script for sub-sampling or rarefaction of data prior to clustering
  • Added script for selecting or removing samples from a de-mulitplexed data set
  • Added script to append an OTU table to meta data file
  • Added support for FUNGuilds functional annotation of taxonomy in OTU table.
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