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smorabit committed Jun 26, 2024
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## TODO:

* Clean up all un-used code
* Clean up all notebooks
* Remove all unused code
* Provide basic comments in all scripts
* In each README section, write a short para or few sentences about the analysis.

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## Differential expression analysis (Figs. 2 and 4)

NoteL:Need to find from Emily's codebase:
Note:Need to find from Emily's codebase:

* Human ST marker genes
* Human ST condition
* Mouse ST marker genes (?)

All snRNA-seq DEG scripts are there

Make a separate script for the Pseudobulk comparisons that we used for the peer review doc?

### snRNA-seq differential expression

TODO: Split the Sex analysis by snRNA vs ST ?
TODO: Split the Sex analysis script by snRNA vs ST ?

* [Marker genes](snRNA/differential_expression/markers/)
* [Disease vs Control DEG scripts](snRNA/differential_expression/condition/)
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## Predicting spatial coordinates for snRNA-seq data (Extended Data Fig. 5)

TODO: cleanup the .Rmd scripts

* [Functions to run CellTrek in parallel for each snRNA-ST pair](snRNA/predict_spatial_coords/celltrek_parallel.R)
* [Running CellTrek in parallel for each snRNA-ST pair](snRNA/predict_spatial_coords/cellrtrek_parallel_run.sub)
* [Downstream plotting script pt. 1](snRNA/predict_spatial_coords/cellrtrek_downsrtream_plotting.Rmd)
* [Downstream plotting script pt. 2](snRNA/predict_spatial_coords/spatial_mapping_celltrek.Rmd)


## Cell-cell communication (CCC) network analysis (Fig. 6)

* [Cell-cell-communication network analysis with CellChat](snRNA/cell_cell_communication/spatial_cellchat.Rmd)
* [Cell-cell-communication network analysis with LIANA and comparing with CellChat](snRNA/cell_cell_communication/LIANA_ccc.Rmd)

## Amyloid-associated gene expression signatures (Fig. 7)

* [Integration of amyloid imaging with ST datasets](spatial/amyloid/splitseq_human_amyloid.Rmd)
* [Human amyloid analysis](spatial/amyloid/voyager_gene_signatures_human.Rmd)
* [Mouse amyloid analysis](spatial/amyloid/voyager_gene_signatures_5x.Rmd)

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