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Query gene expression across BICCN Cross Species Datasets side by side

amahurkar edited this page Apr 17, 2020 · 4 revisions

Janet would like to find out how the expression of her gene of interest compares across multiple species within a specific subtype of neurons. This will entail the following steps:

  1. Select the “Motor Cortex Cross-Species” profile in the dropdown menu on the right-hand side of the NeMO analytics website.
  2. Type the gene of interest into the “Gene Search” box on the left-hand side of the site (user-specified or output from DE/marker analysis).
  3. The established profile provides bar charts for direct comparison between species within cell types.
  4.  Plot expression in tSNE/UMAP
    
  5. Users can alter graphs to their liking by curating the plot, or further analyze the data – both accessible from the top right dropdown menu of a graph window.

Additional option of viewing epigenomics information: 6. Select epigenomic tracks 7. Compare epigenomic data across cell type clusters and species