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danmaclean edited this page Apr 2, 2013 · 44 revisions

Welcome to the ash dieback crowdsource wiki! In this wiki we aim to crowdsource information and genomic resources for Ash Dieback.

So far, the transcriptomes of four infected Ash samples have been sequenced and the transcriptome of unifected Ash:

  • 2 samples of infected Ash from Ashwellthorpe lower wood (The Sainsbury Laboratory)
  • 1 sample of infected Ash from Holt Country Park (The Sainsbury Laboratory)
  • 1 sample of infected Ash from Upton Broad and Marshes (The Sainsbury Laboratory)
  • 1 sample of uninfected Ash from Ashwellthorpe lower wood (The Sainsbury Laboratory)

The genome of a purified culture of Chalara fraxinea isolated from infected Ash collected at Kenninghall Wood has also been sequenced.

Analysis

13 Dec 2012 - AT1 RNA-seq transcripts blastx-ed against eukaryotic protein database show high proportion of fungal enrichment

13 Dec 2012 - Nep1-like proteins (NLPs) identified in AT1 assembled transcripts using tblastn

16 Dec 2012 - How useful is the AT1 assembly?

17 Dec 2012 - Comments on predicted proteins Pfam domains and map to GO, KOG, PRK and EC numbers

20 Dec 2012 - brief comment on pfam for Avr of plant origin

21 Dec 2012 - Phylogenetic tree of Chalaria fraxinea based on ITS / 5.8S rRNA sequences

21 Dec 2012 - AT1 Transcript assemblies - which one?!

14 Jan 2013 - Is the high representation of fungal transcripts in the infected ash material unusual or alarming?

25 Jan 2013 - Analysis of the MAT1 locus of C. fraxinea KW1 isolate

28 Jan 2013 - AT1 and AT2 are of a different mating type to KW1

17 Feb 2013 - Chalara fraxinea gene predictions

18 Mar 2013 - Forest Research Strain mating type info for H.pseudoalbidus and H.albidus