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Welcome to the ash dieback crowdsource wiki! In this wiki we aim to crowdsource information and genomic resources for Ash Dieback.

So far, the transcriptomes of four infected Ash samples have been sequenced and the transcriptome of unifected Ash:

• 2 samples of infected Ash from Ashwellthorpe lower wood (The Sainsbury Laboratory)
• 1 sample of infected Ash from Holt Country Park (The Sainsbury Laboratory)
• 1 sample of infected Ash from Upton Broad and Marshes (The Sainsbury Laboratory)
• 1 sample of uninfected Ash from Ashwellthorpe lower wood (The Sainsbury Laboratory)

The genome of a purified culture of Chalara fraxinea isolated from infected Ash collected at Kenninghall Wood has also been sequenced.

Contributors List of all contributors .. to date!

[Sequence reads](https://github.com/ash-dieback-crowdsource/data/wiki/Sequence reads)

Assemblies

Analysis

April 2014

07 Apr 2014 - Identification of protein coding genes putatively involved in infection by combining metagenomics analysis and protein orthologue clustering

January 2014

27 Jan 2014 - Potential inhibition of a putative alternative oxidase identified in C. fraxinea reduces fungal growth in culture

September 2013

24 Sept 2013 - Variant analysis of different isolates and fruiting bodies of Chalara fraxinea

24 Sept 2013 - Metagenomic analysis to identify species composition of Fraxinus excelsior, Chalara fraxinea and infected material samples

May 2013

26 May 2013 - Compositionally Non Homogenous Domains identified

16 May 2013 - Tree 35 Assembly Stats

03 May 2013 - [Putative alternative oxidase identified in Chalara fraxinea] (https://github.com/ash-dieback-crowdsource/data/wiki/Putative-alternative-oxidase-identified-in-Chalara-fraxinea)

March 2013

18 Mar 2013 - Forest Research Strain mating type info for H.pseudoalbidus and H.albidus

Feb 2013

17 Feb 2013 - Chalara fraxinea gene predictions

January 2013

28 Jan 2013 - AT1 and AT2 are of a different mating type to KW1

25 Jan 2013 - Analysis of the MAT1 locus of C. fraxinea KW1 isolate

14 Jan 2013 - Is the high representation of fungal transcripts in the infected ash material unusual or alarming?

December 2012

21 Dec 2012 - AT1 Transcript assemblies - which one?!

21 Dec 2012 - Phylogenetic tree of Chalaria fraxinea based on ITS / 5.8S rRNA sequences

20 Dec 2012 - brief comment on pfam for Avr of plant origin

17 Dec 2012 - Comments on predicted proteins Pfam domains and map to GO, KOG, PRK and EC numbers

16 Dec 2012 - How useful is the AT1 assembly?

13 Dec 2012 - Nep1-like proteins (NLPs) identified in AT1 assembled transcripts using tblastn

13 Dec 2012 - AT1 RNA-seq transcripts blastx-ed against eukaryotic protein database show high proportion of fungal enrichment

Who is doing what