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Releases: nanoporetech/dorado

v0.9.0

17 Dec 11:45
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[0.9.0] (16 Dec 2024)

This major release of Dorado introduces several new features and enhancements. The polish command, currently experimental, is optimised for refining draft assemblies of human genomes. This release also adds faster DNA modification calling models and improved 6mA false positive rate (FPR) in native human samples. Barcode demultiplexing accuracy has been significantly enhanced for kits with barcodes at both ends, including SQK-NBD114. Note that using custom barcode kits now requires the --kit-name option. A feature has been added to enable running dorado correct in blocks, allowing work to be divided into smaller pieces for easy submission to a compute cluster. Additional updates include the qs tag for mean basecall Q-scores in FASTQ output, an upgrade to POD5 to support systems with large page sizes, improvements to Poly(A) tail length estimation, and various bug fixes to enhance stability and functionality.

  • 2b96c0b - New Dorado polish feature for assembly polishing
  • 0bab166 - Faster modified base models for DNA 4mC_5mC, 5mC_5hmC, 5mCG_5hmCG, and 6mA
  • e637166 - Enable running dorado correct in blocks, for easy submission to a compute cluster
  • 40296da - Reduced false positive classification rates for kits with barcodes at both ends
  • 35da003 - Improve barcode classification when barcodes can be on either end
  • cbcdf38 - Only classify barcodes which are present on sample sheet if provided
  • 2449d03 - Correct AF02F_14 and AH10R_80 barcodes from TWIST-96A-UDI
  • 631e94c - Prevent Dorado demux from stripping alignment information when --no-trim is specified
  • affea85 - Prevent missing filenames when using --emit-summary with Dorado demux
  • 3dec15a - Improve poly(A) tail estimation accuracy, including with interrupted tails
  • df57d34 - Limit poly(A) estimation to reads with plausible signal to prevent stalls in calculation
  • 6cf701a - Add min_primer_separation option to custom poly(A) configuration
  • bf51bd4 - Add qs tag with mean basecall Q-score to FASTQ output
  • dac076d - Upgrade to POD5 v0.3.23 to support systems with large page sizes for POD5 and .fast5
  • c7a7a58 - Prevent silent failure or segfault on Windows with bad custom barcode files
  • 1e829d5 - Do not allow basecalling if target directory includes both POD5 and .fast5 files
  • 05d0981 - Fix modified base trim for reverse-aligned BAM records
  • afdb068 - Fix invalid MM tag after trimming when no mods are present
  • 0d788d7 - Prevent crash when insufficient permissions to read an input file/folder
  • dbece01 - Update custom barcoding documentation to accurately reflect demultiplexing logic
  • 6db40ec - Correct model context info shown in dorado download --list-structured
  • 03acc12 - Use the -o short option only for --output-dir and not for --overlap
  • 8d9c017 - Added support for reading gzipped compressed FASTQ files

v0.7.4

12 Dec 10:49
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[0.7.4] (11 Dec 2024)

This release of Dorado matches the version included in MinKNOW 24.11 and Dorado Basecall Server 7.6.7. We only recommend this release to users who require matching performance to MinKNOW 24.11. Other users should use the latest version of Dorado (≥0.8.3) to benefit from all available improvements. Changes from 0.7.3 in this release include:

  • 40296da - Reduced false positive classifications for NBD barcode kits
  • 687f234 - Improved GPU batch submission timeout logic to improve basecall performance with low data throughput
  • 03e5eec - Fix incorrect basecalls being emitted from SUP models on Apple Silicon
  • 9a81cbc - Fix errors when running dorado basecaller on multi-GPU systems
  • 3cc4de3 - Prevent "Too many open files" error when using --sort-bam with dorado demux
  • f35c8cc - Fix bug when downloading models for dorado correct
  • 4a28d58 - Always trim DNA adapter signal before processing RNA reads
  • adc60ba - Package libcupti.so into ARM Linux builds
  • c65b3fb - Added EXP-NBD114-24 alias for SQK-NBD114-24
  • 69cb260 - Fix bug causing intermittent crashing with v5 SUP models
  • a674dad - Update --help documentation for basecaller, duplex, and correct
  • 6ec77c8 - Fix errors when performing duplex calling with modified bases
  • cb6eee1 - Decouple alignment and inference stages in dorado correct
  • 762e886 - Cache batch sizes to significantly reduce basecaller startup time on supported GPUs
  • 9e5db84 - Fix duplicated alignment tags in re-aligned files
  • 966c2ca - Update POD5 version to v0.3.15
  • e9281fa - Emit an error message if header from input HTS file cannot be read
  • fcb9d53 - Include run name in output files from dorado demux even if input files are FASTQ
  • 7f42b8f - Warn and exit instead of crashing if a model path does not exist
  • 7d74246 - Improve index file error handling
  • 022901e - Fix JSON output when using --list-structured with dorado download

v0.8.3

11 Nov 16:58
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[0.8.3] (11 Nov 2024)

This release of Dorado adds fixes and improvements to the Dorado 0.8.2 release, including a fix to SUP basecalling on Apple Silicon.

  • 03e5eec - Fix incorrect basecalls being emitted from SUP models on Apple Silicon
  • 987a237 - Improve error messaging when unable to run basecalling kernels on Apple GPU

v0.8.2

21 Oct 09:40
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[0.8.2] (21 Oct 2024)

This release of Dorado includes fixes and improvements to the Dorado 0.8.1 release.

  • 9a81cbc - Fix errors when running dorado basecaller on multi-GPU systems
  • bac4469 - Fix error when passing invalid option to basecaller --modified-bases argument
  • 7f40154 - Fix bug when running on GPUs with less than the recommended VRAM
  • a993a32 - Prevent loss of small numbers of reads from dorado correct
  • bbbeb9a - Fix error when dorado demux is provided an empty input directory
  • 3a8094a - Clarify "Unable to find chunk benchmarks" warning to indicate that it is not an error
  • 543ccc1 - Improve documentation on running Dorado in PowerShell on Windows

v0.8.1

04 Oct 09:52
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[0.8.1] (03 Oct 2024)

This release of Dorado includes fixes and improvements to the Dorado 0.8.0 release, including corrected configuration for DNA v5 SUP-compatible 5mC_5hmC and 5mCG_5hmCG models, improved cDNA poly(A) tail estimation for data from MinION flow cells, reduced basecaller startup time on supported GPUs, and more.

  • f74d891 - Corrected bug causing [email protected]_5mC_5hmC@v2 to call CpG contexts only and [email protected]_5mCG_5hmCG@v2 to call all contexts
  • eb46494 - Improve cDNA poly(A) tail estimation for MinION flow cells
  • 762e886 - Cache batch sizes to significantly reduce basecaller startup time on supported GPUs
  • 22269a8 - Prevent "Trim interval is invalid for sequence" error when performing trimming
  • f156ae6 - Prevent write permission error for model download folder when file write is not required
  • fcb9d53 - Include run name in output files from dorado demux even if input files are FASTQ
  • a4c9649 - BED file handling: only split columns on tabs, not spaces; load files with spaces in region names
  • e62cbc8 - Allow comment lines in the middle of the BED file
  • f15c0b3 - Fix compilation in AppleClang 16

v0.8.0

16 Sep 21:18
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[0.8.0] (16 Sept 2024)

This release of Dorado adds v5.1 RNA models with new inosine_m6A and m5C RNA modified base models, updates existing modified base models, improves the speed of v5 SUP basecalling models on A100/H100 GPUs, and enhances the flexibility and stability of dorado correct. It also introduces per-barcode configuration for poly(A) estimation with interrupted tails, adds new --output-dir and --bed-file arguments to Dorado basecalling commands, and includes a variety of other improvements for stability and usability.

  • a69c0a2 - Add v5.1.0 RNA basecalling models, including new inosine_m6A and m5C modified base models, and updated existing DNA and RNA modified base models
  • 8e3a870 - Improve speed of v5 SUP basecalling models on A100 and H100 GPUs
  • 6ee9018 - Reduce false positive calls from v5 DNA modifed base models
  • 69cb260 - Fix bug causing intermittent crashing with v5 SUP models
  • e9dec49 - Add --resume-from functionality to dorado correct
  • cb6eee1 - Decouple alignment and inference stages in dorado correct
  • df861db - Prevent segfaults in dorado correct
  • f35c8cc - Fix bug when downloading models for dorado correct
  • 6646701 - Add per-barcode poly(A) configuration for interrupted tails
  • 0b79407 - Improve poly(A) length estimation for RNA and DNA
  • df614ab - Add --output-dir argument to dorado basecaller and dorado duplex
  • f9beb39 - Add --bed-file argument to dorado basecaller and dorado duplex
  • 1fc6f1e - Add --models-directory option to basecaller, duplex, and download to download and reuse models
  • 966c2ca - Update POD5 version to v0.3.15
  • 6ec77c8 - Fix errors when performing duplex calling with modified bases
  • 4a28d58 - Always trim DNA adapter signal before processing RNA reads
  • a90fbf9 - Fix loading of FASTQ files containing RNA with U bases
  • 9e5db84 - Fix duplicated alignment tags in re-aligned files
  • 3cc4de3 - Prevent "Too many open files" error when using --sort-bam with dorado demux
  • b531918 - Prevent dorado basecaller crash when signal-space trimming removes all raw data
  • adc60ba - Package libcupti.so into ARM Linux builds
  • 667d160 - Remove kit name requirement in custom barcode configuration
  • e9281fa - Emit an error message if header from input HTS file cannot be read
  • 7f42b8f - Warn and exit instead of crashing if a model path does not exist
  • 7d74246 - Improve index file error handling
  • c77733a - Add a mechanism to cache auto batch size calculations
  • a674dad - Update --help documentation for basecaller, duplex, and correct
  • 022901e - Fix JSON output when using --list-structured with dorado download
  • db73e5d - Add run_id to filenames output by demux

v0.7.3

01 Aug 15:14
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[0.7.3] (1 Aug 2024)

This release of Dorado updates dorado correct to fix handling of high copy repeats and avoid shutdown hanging. It also includes dorado demux improvements to reduce false matches in midstrand barcode detection and ensure correct file naming, along with other fixes.

  • 5dc78ab - Remove limit on number of overlaps considered during all-vs-all alignment in dorado correct
  • 2741de7 - Prevent hang during shutdown of dorado correct and prevent out of memory errors
  • 37d316c - Remove unused --read-ids and --threads parameters from dorado correct
  • ddb13de - Increase the threshold for midstrand barcode detection to reduce false matches
  • 845a3ad - Fix misnaming by dorado demux of barcode file for barcodes ending in a letter (e.g., 12a)
  • 56d3e8e - Fix seq/qual orientation when demultiplexing aligned BAMs
  • 5ddfc2f - Fix bug causing CUDA illegal memory access with v5 RNA SUP and mods

v0.6.3

01 Aug 09:45
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[0.6.3] (31 July 2024)

This release matches the version of Dorado in MinKNOW 24.06 and Dorado Basecall Server 7.4.12.

  • 29f311b - Increase the threshold for midstrand barcode detection to reduce false matches
  • 01735dd - Fix misnaming by dorado demux of barcode file for barcodes ending in a letter (e.g., 12a)
  • c65f1f3 - Fixes to support live basecalling in MinKNOW

v0.7.2

18 Jun 10:29
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[0.7.2] (18 June 2024)

This release of Dorado resolves basecalling failures when running v5 SUP models on CPU-only devices or v5 RNA HAC on Apple silicon. It also fixes bugs in dorado demux and dorado correct, and corrects sm and sd tags to match the Dorado SAM specification.

  • 3835272 - Fix bug causing v5 SUP models to fail when running on CPU-only devices
  • c36f444 - Fix bug causing RNA v5 HAC basecalling to fail on Apple silicon
  • 3621800 - Fix bug causing segfault in dorado demux
  • 3b51c1b - Fix sub-par alignments in dorado correct
  • d0df79c - Correct shift and scale (sm and sd) SAM tags to match SAM specification

v0.7.1

04 Jun 02:46
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[0.7.1] (3 June 2024)

This release of Dorado fixes out of memory errors when using the v5 SUP model with methylation calling, resolves several bugs in dorado correct and adds correct handling of the BC:Z tag when running demux multiple times.

  • a9c6f59 - Fetch available memory correctly for autobatch calculation with modbase models
  • eb24124 - Move developer quickstart and extend installation instructions
  • 45b8acc - Package missing CUDA Toolkit dependencies with dorado
  • 33578e7 - Update BC tag instead of adding a new one
  • 580ad61 - Prevent creation of CUDA stream when device is CPU
  • 82078c5 - Fix segfault with htslib pointer freeing in Windows