-
Notifications
You must be signed in to change notification settings - Fork 11
ngs_FASTQC
Stephen Fisher edited this page Jan 20, 2015
·
5 revisions
This module will run FastQC on the specified fastq files.
Usage: ngs.sh fastqc [-i inputDir] [-o outputDir] [-se] sampleID Input: sampleID/inputDir/unaligned_1.fq sampleID/inputDir/unaligned_2.fq (paired-end reads) Output: sampleID/outputDir/SAMPLEID.read_1.outputDir.html sampleID/outputDir/SAMPLEID.read_2.outputDir.html (paired-end reads) Requires: FastQC ( http://www.bioinformatics.babraham.ac.uk/projects/fastqc/ ) Options: -i inputDir - location of source file (default: init). -i outputDir - location of output files (default: fastqc). If this is changed from the default, then it will not be accessible by the STATS module. -se - single-end reads (default: paired-end)
Run FastQC on sampleID/inputDir/unaligned_1.fq and if PE also on sampleID/inputDir/unaligned_2.fq. FastQC only uses one input file so it is run twice in the case of pair-end reads.