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ngs_RUM
Stephen Fisher edited this page Mar 11, 2014
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This module will run the RUM aligner. The RUM alignment program must be installed separately (see Requires below).
Usage: ngs.sh rum [-i inputDir] -p numProc -s species [-se] sampleID Input: sampleID/INPUTDIR/unaligned_1.fq sampleID/INPUTDIR/unaligned_2.fq (paired-end reads) Output: sampleID/rum/sampleID.rum.sorted.bam (all aligned reads) sampleID/rum/sampleID.rum.unique.bam (uniquely aligned reads) Requires: RUM ( http://cbil.upenn.edu/RUM ) samtools ( http://samtools.sourceforge.net/ ) Options: -i inputDir - location of source files (default: trim). -p numProc - number of cpu to use. -s species - species from repository: /lab/repo/resources/rum2. -se - single-end reads (default: paired-end)
Runs RUM using the trimmed files from sampleID/trim. Output is stored in sampleID/rum directory. No non-default options are specified for RUM.