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ngs_SNP
Stephen Fisher edited this page Feb 28, 2014
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Run SNP calling on the sorted bam file (ie sampleID/bowtie).
Usage: ngs.sh snp [-i inputDir] -s species sampleID Input: sampleID/inputDir/sampleID.sorted.bam Output: sampleID/snp/sampleID.filtered.vcf sampleID/snp/sampleID.bigWig Requires: freebayes ( https://github.com/ekg/freebayes ) bedtools ( http://bedtools.readthedocs.org/en/latest/ ) Kent sources ( http://genomewiki.ucsc.edu/index.php/Kent_source_utilities ) Options: -i inputDir - directory with unaligned reads (default: bowtie) -s species - species from repository: /lab/repo/resources/snp
Run SNP calling on the sorted bam file (ie sampleID/bowtie). Output is placed in the directory sampleID/snp.